Formation and Expansion of Neurospheres from a Hippocampal Tissue

Source: Soares, R. et al., Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches. J. Vis. Exp. (2020). This video demonstrates the process of neurosphere formation and their expansion from hippocampal tissue. Neural stem and progenitor cells from the postnatal mouse dentate gyrus tissue proliferate and form primary neurospheres, which are then reseeded to form secondary neurospheres.