Characterizing Extracellular Space in a Brain Slice Using Ion-Selective and Iontophoresis Microelectrodes

Source:  Odackal, J., et al. Real-time iontophoresis with tetramethylammonium to quantify volume fraction and tortuosity of brain extracellular space. J. Vis. Exp. (2017) This video demonstrates how to characterize the extracellular space in a brain slice by using ion-selective and iontophoresis microelectrodes to measure and compare electrical signals. This process helps determine the space's volume fraction and degree of twisting.