Calcium Imaging in Individual Neurons Regulating Egg-Laying Behavior in <em>Caenorhabditis elegans</em>

Source: Ravi, B. et al., Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans. J. Vis. Exp. (2018). This video demonstrates a technique for calcium imaging in individual neurons of behaving Caenorhabditis elegans using genetically encoded fluorescent reporter proteins. It outlines the steps involved in imaging worm movement and measuring reporter fluorescence ratios from neurons to correlate intracellular calcium levels with neuronal activity and behavioral changes during egg-laying.