Trans-Spinal Direct Current Stimulation of a Rat's Spinal Motoneuron

Source: Bączyk, M., et al. In Vivo Intracellular Recording of Type-Identified Rat Spinal Motoneurons During Trans-Spinal Direct Current Stimulation. J. Vis. Exp. (2020). This video describes the in vivo intracellular recording of rat motoneurons under trans-spinal direct current stimulation. The protocol describes how to measure membrane properties and record the rhythmic firing of motoneurons before, during, and after anodal or cathodal polarization of the spinal cord.