Ultrastructural Analysis of a Mouse Brain Section Using Transmission Electron Microscopy

Source: Lutz, D., et.al. Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord. J. Vis. Exp. (2019).This video demonstrates the preparation and transmission electron microscopy (TEM) analysis of a brain section from a mouse pup. The protocol starts with osmium tetroxide-fixed tissue embedded in resin, followed by precise trimming to isolate the region of interest and ultramicrotomy to produce semithin and ultrathin sections, which are visualized using light microscopy and TEM, respectively.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31506
Primary Topic
Electron and X-Ray Spectroscopy Techniques
Type
article
Field-Weighted Citation Impact
0.00
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article

Ultrastructural Analysis of a Mouse Brain Section Using Transmission Electron Microscopy

Journal of Visualized Experiments
Electron and X-Ray Spectroscopy Techniques
article

Ultrastructural Analysis of a Mouse Brain Section Using Transmission Electron Microscopy

article en

Abstract

Source: Lutz, D., et.al. Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord. J. Vis. Exp. (2019).This video demonstrates the preparation and transmission electron microscopy (TEM) analysis of a brain section from a mouse pup. The protocol starts with osmium tetroxide-fixed tissue embedded in resin, followed by precise trimming to isolate the region of interest and ultramicrotomy to produce semithin and ultrathin sections, which are visualized using light microscopy and TEM, respectively.

Journal of Visualized Experiments
Openalex Percentile: Top 83%
Electron and X-Ray Spectroscopy Techniques
DOISourceOpenAlex
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Ultrastructural Analysis of a Mouse Brain Section Using Transmission Electron Microscopy · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS