Immunostaining of Fluorescently Labeled Astrocytes in a Mouse Brain Tissue Section
Source: Eaker, A. R., et al. Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections. J. Vis. Exp. (2022) This video demonstrates the procedure for immunostaining a fixed mouse brain tissue section with fluorescently labeled astrocytes using primary and secondary antibodies and capture the images of the stained astrocytes through confocal microscopy.
Publication Details
- Journal
- Journal of Visualized Experiments
- Published
- 2025-07-24
- DOI
- https://doi.org/10.3791/31433
- Primary Topic
- Immune cells in cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00