Immunostaining of Fluorescently Labeled Astrocytes in a Mouse Brain Tissue Section

Source: Eaker, A. R., et al. Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections. J. Vis. Exp. (2022) This video demonstrates the procedure for immunostaining a fixed mouse brain tissue section with fluorescently labeled astrocytes using primary and secondary antibodies and capture the images of the stained astrocytes through confocal microscopy.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31433
Primary Topic
Immune cells in cancer
Type
article
Field-Weighted Citation Impact
0.00
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article

Immunostaining of Fluorescently Labeled Astrocytes in a Mouse Brain Tissue Section

Journal of Visualized Experiments
Immune cells in cancer
article

Immunostaining of Fluorescently Labeled Astrocytes in a Mouse Brain Tissue Section

article en

Abstract

Source: Eaker, A. R., et al. Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections. J. Vis. Exp. (2022) This video demonstrates the procedure for immunostaining a fixed mouse brain tissue section with fluorescently labeled astrocytes using primary and secondary antibodies and capture the images of the stained astrocytes through confocal microscopy.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 80%
Immune cells in cancer
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Immunostaining of Fluorescently Labeled Astrocytes in a Mouse Brain Tissue Section · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS