Immunoprecipitation of Target Protein-DNA Complexes from Motor Neuron Lysate

Source: Montanera, K. N. et al., Rhee, H. S. High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo). J. Vis. Exp. (2020) This video demonstrates the immunoprecipitation of DNA fragments crosslinked with target protein from stem cell-derived motor neuron lysates using target protein-specific antibody-coated magnetic beads.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31309
Primary Topic
RNA Interference and Gene Delivery
Type
article
Field-Weighted Citation Impact
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article

Immunoprecipitation of Target Protein-DNA Complexes from Motor Neuron Lysate

Journal of Visualized Experiments
RNA Interference and Gene Delivery
article

Immunoprecipitation of Target Protein-DNA Complexes from Motor Neuron Lysate

article en

Abstract

Source: Montanera, K. N. et al., Rhee, H. S. High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo). J. Vis. Exp. (2020) This video demonstrates the immunoprecipitation of DNA fragments crosslinked with target protein from stem cell-derived motor neuron lysates using target protein-specific antibody-coated magnetic beads.

Journal of Visualized Experiments
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Openalex Percentile: Top 83%
RNA Interference and Gene Delivery
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Immunoprecipitation of Target Protein-DNA Complexes from Motor Neuron Lysate · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS