Measuring the Infarct Zone and Brain Edema in a Rat Brain Following Middle Cerebral Artery Occlusion

Source: Frank, D., et al. Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples. J. Vis. Exp. (2020). This video demonstrates the assessment of the infarct zone and brain edema in brain slices from rats subjected to middle cerebral artery occlusion. Infarcted areas are identified using redox staining, while a blue filter and threshold function in image analysis software quantify the infarct zone and edema in the affected hemisphere.