Labeling of Biocytin-Filled Interneurons in Rat Hippocampal Slices for Visualization

Source: Economides, G., et al. Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons. J. Vis. Exp. (2018) This video demonstrates the procedure for staining fixed rat brain slices containing biocytin-filled interneurons. The protocol includes incubation with avidin-biotin complex (ABC), staining with HRP and chromogenic substrate, preserving structures with osmium tetroxide, and embedding the tissue in resin for imaging.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31416
Primary Topic: Cell Image Analysis Techniques
Type: article
Field-Weighted Citation Impact: 0.00

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article

Labeling of Biocytin-Filled Interneurons in Rat Hippocampal Slices for Visualization

Jul 24, 2025

Journal of Visualized Experiments

Cell Image Analysis Techniques

article

Labeling of Biocytin-Filled Interneurons in Rat Hippocampal Slices for Visualization

July 24, 2025article en

Abstract

Journal of Visualized Experiments

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Openalex Percentile: Top 87%

Cell Image Analysis Techniques

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Labeling of Biocytin-Filled Interneurons in Rat Hippocampal Slices for Visualization · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS