Detecting Hydrogen Peroxide Levels in Cultured Neurons Through Live-Cell Fluorescence Imaging

Source: Terzi, A. et al., ROS Live Cell Imaging During Neuronal Development. J. Vis. Exp. (2021). This video demonstrates a technique to detect real-time hydrogen peroxide levels in retinal ganglion cells using the roGFP2-Orp1 biosensor and live cell imaging techniques. It outlines the steps involved in cell preparation, fluorescence imaging, and the quantification of hydrogen peroxide levels.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31174
Primary Topic
Photoreceptor and optogenetics research
Type
article
Field-Weighted Citation Impact
0.00
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Detecting Hydrogen Peroxide Levels in Cultured Neurons Through Live-Cell Fluorescence Imaging

1 links
Journal of Visualized Experiments
Photoreceptor and optogenetics research
article

Detecting Hydrogen Peroxide Levels in Cultured Neurons Through Live-Cell Fluorescence Imaging

article en

Abstract

Source: Terzi, A. et al., ROS Live Cell Imaging During Neuronal Development. J. Vis. Exp. (2021). This video demonstrates a technique to detect real-time hydrogen peroxide levels in retinal ganglion cells using the roGFP2-Orp1 biosensor and live cell imaging techniques. It outlines the steps involved in cell preparation, fluorescence imaging, and the quantification of hydrogen peroxide levels.

Journal of Visualized Experiments
Openalex Percentile: Top 80%
Photoreceptor and optogenetics research
DOISourceOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.

Detecting Hydrogen Peroxide Levels in Cultured Neurons Through Live-Cell Fluorescence Imaging · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS