Preparing Cultured Neurons for Optical Analysis of Recycled Synaptic Vesicles

Source: Riemann, D. et al., An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins. J. Vis. Exp. (2018) This video demonstrates the procedure for preparing cultured neurons for optical analysis of recycled synaptic vesicles. The process involves fluorescence tagging of synaptic vesicle proteins and immunostaining to label and visualize the recycled vesicles within the neurons.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31345
Primary Topic
Neuroscience and Neural Engineering
Type
article
Field-Weighted Citation Impact
0.00
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article

Preparing Cultured Neurons for Optical Analysis of Recycled Synaptic Vesicles

Journal of Visualized Experiments
Neuroscience and Neural Engineering
article

Preparing Cultured Neurons for Optical Analysis of Recycled Synaptic Vesicles

article en

Abstract

Source: Riemann, D. et al., An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins. J. Vis. Exp. (2018) This video demonstrates the procedure for preparing cultured neurons for optical analysis of recycled synaptic vesicles. The process involves fluorescence tagging of synaptic vesicle proteins and immunostaining to label and visualize the recycled vesicles within the neurons.

Journal of Visualized Experiments
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Openalex Percentile: Top 80%
Neuroscience and Neural Engineering
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Preparing Cultured Neurons for Optical Analysis of Recycled Synaptic Vesicles · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS