Analyzing the Glutamate Release and Clearance at a Single Neuron Synapse in a Mouse Brain Slice

Source: Dvorzhak, A. et al., Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice. J. Vis. Exp. (2020) This video demonstrates evaluating glutamate release and clearance at single corticostriatal synapses in a mouse brain slice. Electrical stimulation facilitates glutamate release at the synapse. The released glutamate alters sensor protein's fluorescence, which decays over time, indicating release and clearance efficiency.