Obtaining a Single-Cell Suspension from Mouse Hippocampal Tissue

Source: Trujillo, M., et al. Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue. J. Vis. Exp. (2021) This video demonstrates a protocol to obtain a highly viable single-cell suspension from the mouse brain hippocampus using a combination of automated mechanical dissociation and a mixture of enzymes to aid in enzymatic digestion.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31113
Primary Topic: Pluripotent Stem Cells Research
Type: article
Field-Weighted Citation Impact: 0.00

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article

Obtaining a Single-Cell Suspension from Mouse Hippocampal Tissue

Jul 24, 2025

Journal of Visualized Experiments

Pluripotent Stem Cells Research

article

Obtaining a Single-Cell Suspension from Mouse Hippocampal Tissue

July 24, 2025article en

Abstract

Journal of Visualized Experiments

Openalex Percentile: Top 83%

Pluripotent Stem Cells Research

DOI Source OpenAlex

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Obtaining a Single-Cell Suspension from Mouse Hippocampal Tissue · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS