Developing Human iPSC-Derived Motor Nerve Organoids Using a Microfluidic Chip

Source: Osaki, T., et al. Three-Dimensional Motor Nerve Organoid Generation. J. Vis. Exp. (2020). This video demonstrates the method of creating motor nerve organoids by first culturing human induced pluripotent stem cells in a special medium to form spheroids, then differentiating them into motor neurons using specific inhibitors, and finally cultivating them in a microfluidic chip to extend axons and form motor nerve organoids.