Enhancing Stem Cell Differentiation with a Transient DMSO Treatment

Source: Sambo, D. et al., Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation. J. Vis. Exp. (2019) This video demonstrates the method for enhancing stem cell differentiation with transient DMSO treatment, which arrests cells in the G1 phase. Post-treatment, an ectodermal differentiation medium with BMP and TGF-β inhibitors is added to promote neural precursor formation.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31110
Primary Topic
Pluripotent Stem Cells Research
Type
article
Field-Weighted Citation Impact
0.00
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article

Enhancing Stem Cell Differentiation with a Transient DMSO Treatment

Journal of Visualized Experiments
Pluripotent Stem Cells Research
article

Enhancing Stem Cell Differentiation with a Transient DMSO Treatment

article en

Abstract

Source: Sambo, D. et al., Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation. J. Vis. Exp. (2019) This video demonstrates the method for enhancing stem cell differentiation with transient DMSO treatment, which arrests cells in the G1 phase. Post-treatment, an ectodermal differentiation medium with BMP and TGF-β inhibitors is added to promote neural precursor formation.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 83%
Pluripotent Stem Cells Research
DOISourceOpenAlex
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Enhancing Stem Cell Differentiation with a Transient DMSO Treatment · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS