Detection of Orexin and Cannabinoid Receptor Co-localization in Diet-Induced Obese Zebrafish

Source: Imperatore, R., et al. Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods. J. Vis. Exp. (2019). This video demonstrates a protocol for investigating the co-localization of orexin and cannabinoid receptors in normal and diet-induced obesity (DIO) adult zebrafish brain tissue using immunofluorescence.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31374
Primary Topic
Sleep and Wakefulness Research
Type
article
Field-Weighted Citation Impact
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article

Detection of Orexin and Cannabinoid Receptor Co-localization in Diet-Induced Obese Zebrafish

Journal of Visualized Experiments
Sleep and Wakefulness Research
article

Detection of Orexin and Cannabinoid Receptor Co-localization in Diet-Induced Obese Zebrafish

article en

Abstract

Source: Imperatore, R., et al. Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods. J. Vis. Exp. (2019). This video demonstrates a protocol for investigating the co-localization of orexin and cannabinoid receptors in normal and diet-induced obesity (DIO) adult zebrafish brain tissue using immunofluorescence.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 82%
Sleep and Wakefulness Research
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Detection of Orexin and Cannabinoid Receptor Co-localization in Diet-Induced Obese Zebrafish · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS