Measuring Odorant Receptor Activation using a Real-Time Cyclic Adenosine Monophosphate Assay

Source: Zhang, Y. et al., Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay. J. Vis. Exp.(2017) This video demonstrates the protocol for measuring odorant receptor activation in transfected cells using a cyclic adenosine monophosphate (cAMP) biosensor and luminescence detection. The method is applied to study receptor-ligand interactions and signaling pathways in olfactory neurons.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31351
Primary Topic: Olfactory and Sensory Function Studies
Type: article
Field-Weighted Citation Impact: 0.00

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article

Measuring Odorant Receptor Activation using a Real-Time Cyclic Adenosine Monophosphate Assay

Jul 24, 2025

Journal of Visualized Experiments

Olfactory and Sensory Function Studies

article

Measuring Odorant Receptor Activation using a Real-Time Cyclic Adenosine Monophosphate Assay

July 24, 2025article en

Abstract

Journal of Visualized Experiments

Openalex Percentile: Top 82%

Olfactory and Sensory Function Studies

DOI Source OpenAlex

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Measuring Odorant Receptor Activation using a Real-Time Cyclic Adenosine Monophosphate Assay · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS