Optical Recording of Neuronal Activity in Brain Slices Stained with a Voltage-Sensitive Dye

Source: Tominaga, Y., et al. Wide-field Single-photon Optical Recording in Brain Slices Using Voltage-sensitive Dye. J. Vis. Exp. (2019). This video demonstrates a procedure for observing changes in neuronal activity in a brain slice stained with a voltage-sensitive dye. The dye reflects changes in the neuron's membrane potential by altering its fluorescence intensity. Through the application of electrical stimuli and advanced imaging techniques, this process enables visual tracking of real-time changes in neuronal membrane potential based on VSD fluorescence.