Assessing a Functional Neuromuscular Junction Via Simultaneous Optical Stimulation and Video Recording

Source:  Liberman, M., et al., Engineering and Characterization of an Optogenetic Model of the Human Neuromuscular Junction. J. Vis. Exp. (2022). This video demonstrates a method to assess functional neuromuscular junctions. Using a bioreactor containing muscle tissue and motor neurons, along with fluorescent microscopy, the light-sensitive channels in the neurons are stimulated to generate electrical signals, causing muscle fiber contractions.