Immunohistochemistry for Identifying the Locus Coeruleus Region in Mouse Brain Sections

Source: Schmidt, K. et al., Localization of the Locus Coeruleus in the Mouse Brain. J. Vis. Exp. (2019) This protocol outlines the use of immunohistochemistry to identify locus coeruleus regions in mouse brain sections. By targeting dopamine β-hydroxylase with green fluorophore-labeled antibodies, norepinephrine-producing neurons are visualized as green fluorescence under a microscope.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31401
Primary Topic
Congenital heart defects research
Type
article
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article

Immunohistochemistry for Identifying the Locus Coeruleus Region in Mouse Brain Sections

Journal of Visualized Experiments
Congenital heart defects research
article

Immunohistochemistry for Identifying the Locus Coeruleus Region in Mouse Brain Sections

article en

Abstract

Source: Schmidt, K. et al., Localization of the Locus Coeruleus in the Mouse Brain. J. Vis. Exp. (2019) This protocol outlines the use of immunohistochemistry to identify locus coeruleus regions in mouse brain sections. By targeting dopamine β-hydroxylase with green fluorophore-labeled antibodies, norepinephrine-producing neurons are visualized as green fluorescence under a microscope.

Journal of Visualized Experiments
Zero hunger
Openalex Percentile: Top 83%
Congenital heart defects research
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Immunohistochemistry for Identifying the Locus Coeruleus Region in Mouse Brain Sections · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS