Quantification of Filamentous Actin Puncta in Rat Cortical Neurons

Source: Li, H., et al. Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons. J. Vis. Exp. (2016) This video demonstrates the procedure for fluorescently staining rat cortical neurons for microscopy. It targets F-actin and dendritic proteins to analyze structural integrity and calculate F-actin density, which is crucial for studying neuronal function and health.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31303
Primary Topic
Cellular Mechanics and Interactions
Type
article
Field-Weighted Citation Impact
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article

Quantification of Filamentous Actin Puncta in Rat Cortical Neurons

Journal of Visualized Experiments
Cellular Mechanics and Interactions
article

Quantification of Filamentous Actin Puncta in Rat Cortical Neurons

article en

Abstract

Source: Li, H., et al. Quantification of Filamentous Actin (F-actin) Puncta in Rat Cortical Neurons. J. Vis. Exp. (2016) This video demonstrates the procedure for fluorescently staining rat cortical neurons for microscopy. It targets F-actin and dendritic proteins to analyze structural integrity and calculate F-actin density, which is crucial for studying neuronal function and health.

Journal of Visualized Experiments
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Openalex Percentile: Top 80%
Cellular Mechanics and Interactions
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Quantification of Filamentous Actin Puncta in Rat Cortical Neurons · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS