Recording Gamma Band Oscillations From Pedunculopontine Nucleus Neurons in a Brain Slice

Source: Urbano, F. J., et al. Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons. J. Vis. Exp. (2016). The video demonstrates the method to record gamma-band oscillations in PPN neurons. Using a sagittal brain section with synaptic blockers in aCSF, it employs positive current pulses via a patch pipette to open calcium channels, inducing crucial subthreshold activity for wakefulness.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31199
Primary Topic
Neural dynamics and brain function
Type
article
Field-Weighted Citation Impact
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article

Recording Gamma Band Oscillations From Pedunculopontine Nucleus Neurons in a Brain Slice

Journal of Visualized Experiments
Neural dynamics and brain function
article

Recording Gamma Band Oscillations From Pedunculopontine Nucleus Neurons in a Brain Slice

article en

Abstract

Source: Urbano, F. J., et al. Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons. J. Vis. Exp. (2016). The video demonstrates the method to record gamma-band oscillations in PPN neurons. Using a sagittal brain section with synaptic blockers in aCSF, it employs positive current pulses via a patch pipette to open calcium channels, inducing crucial subthreshold activity for wakefulness.

Journal of Visualized Experiments
Openalex Percentile: Top 82%
Neural dynamics and brain function
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Recording Gamma Band Oscillations From Pedunculopontine Nucleus Neurons in a Brain Slice · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS