Modeling Retinal Degeneration and Regeneration in Zebrafish Using a Focal Laser Injury

Source: Conedera, F. M., et al. Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish. J. Vis. Exp. (2017). This video demonstrates a procedure to induce retinal degeneration in adult zebrafish using laser injury. The laser targets photoreceptor cells in the retina, triggering cell death and a degenerative process. This damage activates Müller glia (MG) cells, leading to their dedifferentiation into stem cell progenitors that aid in retinal regeneration.