Generation of Neuronal Cells from Blood-Derived Pluripotent Stem Cells

Source: Becker-Kojić, Z. A., et al. GM-Free Generation of Blood-Derived Neuronal Cells. J. Vis. Exp. (2021). This video demonstrates a technique to generate neuronal cells from blood-derived pluripotent stem cells (BD-PSCs). The BD-PSCs are obtained via reprogramming blood progenitor cells. The undifferentiated BD-PSCs are cultured on cell culture substrate-coated coverslips using a neural induction medium and a neural differentiation medium, triggering differentiation into cells with a neuronal phenotype.