Fluorescence-Based Monitoring of Mitochondrial Stress in Astrocytes

Source: Espourteille, J., et al. Live-imaging of Mitochondrial System in Cultured Astrocytes. J. Vis. Exp. (2021) This video demonstrates the evaluation of mitochondrial health in genetically modified astrocytes, which express a fluorescent protein within their mitochondria. The protein helps to track changes in mitochondrial health as its fluorescence changes from green to red, indicating stress or damage. Images are captured and analyzed using various parameters.