Differentiation and Maturation of Human Embryonic Stem Cells into Neural Organoids

Source: Cosset, E., et al. Human neural organoids for studying brain cancer and neurodegenerative diseases. J. Vis. Exp. (2019) In this video, a stepwise protocol for Human embryonic stem cells into neural organoids is demonstrated. The method involves inducing neuronal rosette formation, followed by controlled maturation and final culturing on a hydrophobic membrane to achieve fully developed neural organoids.