Implantation of an Electrode Assembly in a Mouse Brain for Electrophysiological Recording

Source: Straub, J., et al. Preparation and Implantation of Electrodes for Electrically Kindling VGAT-Cre Mice to Generate a Model for Temporal Lobe Epilepsy. J. Vis. Exp. (2021). This video demonstrates a technique for implanting an electrode assembly into a mouse brain. The assembly contains bipolar electrodes to record activity from the hippocampus and a reference electrode to obtain baseline activity from the cerebellum. An anesthetized mouse is placed on a stereotaxic frame, and the electrode assembly is implanted in the target brain region. The animal is then allowed to recover before performing electrophysiological recording.