Isolating and Culturing Cells from Diffuse Intrinsic Pontine Glioma

Source: Lin, G. L. et al., A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG). J. Vis. Exp. (2017) This video demonstrates the process of isolating and culturing glial progenitor cells from diffuse intrinsic pontine glioma, or DIPG-affected brainstem tissue. Glial cells isolated from tumor tissue via an enzymatic digestion are cultured in a neurobasal medium, which promotes their growth and the development of free-floating neurospheres.