Obtaining Horizontal Mouse Hippocampal Brain Slices

Source: Van Hoeymissen, E., et al. Horizontal Hippocampal Slices of the Mouse Brain. J. Vis. Exp. (2020) This video demonstrates a detailed protocol for preparing horizontal hippocampal brain slices from mice, focusing on preserving the integrity of hippocampal fiber pathways. The process involves carefully bisecting and securing the brain, using a vibratome for slicing, and maintaining optimal tissue conditions with a carbogenated fluid, resulting in hippocampal brain slices ready for further experimentation.