Fluorescence Immunostaining of Cerebellar Organoid Slices

Source: Silva, T. P., et al., Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining. J. Vis. Exp. (2020) This video demonstrates the immunofluorescence staining of cerebellar organoid slices to identify specific target antigens expressed in various cerebellar neurons, which are indicative of organoid maturation.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31310
Primary Topic
Radiopharmaceutical Chemistry and Applications
Type
article
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article

Fluorescence Immunostaining of Cerebellar Organoid Slices

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Journal of Visualized Experiments
Radiopharmaceutical Chemistry and Applications
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Fluorescence Immunostaining of Cerebellar Organoid Slices

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Abstract

Source: Silva, T. P., et al., Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining. J. Vis. Exp. (2020) This video demonstrates the immunofluorescence staining of cerebellar organoid slices to identify specific target antigens expressed in various cerebellar neurons, which are indicative of organoid maturation.

Journal of Visualized Experiments
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Radiopharmaceutical Chemistry and Applications
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Fluorescence Immunostaining of Cerebellar Organoid Slices · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS