Preparing a Single-Cell Suspension of Immune Cells from Murine Brain Tissue

Source: DiSano, K. D., et al. Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune Cells. J. Vis. Exp. (2020). The video demonstrates the preparation of a single-cell suspension of immune cells from a murine brain tissue. It involves enzymatic digestion to release cells from the brain tissue, followed by density gradient centrifugation to isolate the immune cells based on their densities to obtain the single-cell suspension.