Establishing a Whole-Cell Configuration through the Patch Clamp Method

Source: Segev, A. et al., Whole-cell Patch-clamp Recordings in Brain Slices. J. Vis. Exp. (2016) This video demonstrates the whole-cell patch clamp technique in a mouse brain slice. This technique establishes a stable connection between micropipette and neuron membrane, which is further disrupted to establish a whole-cell configuration to study the neuron's health and physiological responses.