Protein Extraction from Neuronal Cells

Source: Shani, T., et al. Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells. J. Vis. Exp. (2017) This protocol outlines the extraction of proteins from neuronal cancer cells. The cells are washed with a buffer and lysed via enzymatic and mechanical disruption. The extracted protein-rich supernatant is collected after centrifugation.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31297
Primary Topic
Genetics, Bioinformatics, and Biomedical Research
Type
article
Field-Weighted Citation Impact
0.00
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article

Protein Extraction from Neuronal Cells

Journal of Visualized Experiments
Genetics, Bioinformatics, and Biomedical Research
article

Protein Extraction from Neuronal Cells

article en

Abstract

Source: Shani, T., et al. Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells. J. Vis. Exp. (2017) This protocol outlines the extraction of proteins from neuronal cancer cells. The cells are washed with a buffer and lysed via enzymatic and mechanical disruption. The extracted protein-rich supernatant is collected after centrifugation.

Journal of Visualized Experiments
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Openalex Percentile: Top 83%
Genetics, Bioinformatics, and Biomedical Research
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Protein Extraction from Neuronal Cells · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS