Determining Nanoparticle-Induced Toxicity in Neurons Using Microglia as a Surrogate Biosensor

Source: Duffy, C. M. et al., Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity. J. Vis. Exp. (2016). This video demonstrates a method to assess the neurotoxic effects of nanoparticles using microglia. It outlines the steps for activating microglia with nanoparticles, filtering conditioned media to isolate microglia-derived pro-inflammatory cytokines, and testing hypothalamic neuron toxicity with the conditioned media.