Differentiating Human Embryonic Stem Cells into Neural Progenitors

Source: Jeon, K. et al., Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells. J. Vis. Exp. (2020) This video demonstrates the differentiation of human embryonic stem cells, or hESCs, directly into neural progenitor cells without using feeder cells or any intermediate steps. The selective inhibition of bone morphogenetic protein or BMP and transforming growth factor-beta, or TGF-β, signaling pathways leads to hESCs differentiation into neural progenitor cells, or NPCs.