Development and Maintenance of Serum-Free Embryoid Bodies

Source: Phillips, A. W., et al. Developing HiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays. J. Vis. Exp. (2017). The video describes the procedure for developing serum-free embryoid bodies (SFEBs) from neuronal progenitor cells using various serum-free differentiation mediums or DM. DM-2 facilitates neurons' maturation and arrangement to form SFEBs, while DM-3 is used for further growth maintenance.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31161
Primary Topic
Reproductive Biology and Fertility
Type
article
Field-Weighted Citation Impact
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article

Development and Maintenance of Serum-Free Embryoid Bodies

Journal of Visualized Experiments
Reproductive Biology and Fertility
article

Development and Maintenance of Serum-Free Embryoid Bodies

article en

Abstract

Source: Phillips, A. W., et al. Developing HiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays. J. Vis. Exp. (2017). The video describes the procedure for developing serum-free embryoid bodies (SFEBs) from neuronal progenitor cells using various serum-free differentiation mediums or DM. DM-2 facilitates neurons' maturation and arrangement to form SFEBs, while DM-3 is used for further growth maintenance.

Journal of Visualized Experiments
Openalex Percentile: Top 74%
Reproductive Biology and Fertility
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Development and Maintenance of Serum-Free Embryoid Bodies · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS