Isolation and Culture of Primary Neurons and Glia from a Rat Urinary Bladder

Source: Wang, R. et al., Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder. J. Vis. Exp. (2020). This video demonstrates a technique for generating a primary culture of neurons and glia from a rat's urinary bladder. It outlines the steps involved in tissue preparation, enzymatic digestion to obtain single cells, and culturing the cells in a neuron-specific medium to establish a primary neuronal and glial culture.