Analyzing Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures

Source: Rakotomamonjy, J., et al. Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures. J. Vis. Exp. (2019) This video illustrates a technique for obtaining cerebellar organotypic slices from a mouse pup's brain and culturing the slices in media supplemented with nutrients and growth factors to mimic cerebellar development in vitro. Additionally, specific cerebellar slices are treated with a test compound, and the compound's neuroprotective ability is analyzed using an immunofluorescence staining technique.