Differentiating Human-Induced Pluripotent Stem Cells into Brain Microvascular Endothelial Cells

Source: Pong, S. et al., Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (2020) This video demonstrates a multi-step process for differentiating human iPSCs into brain microvascular endothelial cells. The resulting differentiated cells closely mimic brain microvascular endothelial cells, characterized by tight junctions, making them ideal for research on brain diseases.