Establishing a Whole-Cell Configuration for Two-Photon Calcium Imaging of Brain Slices

Source: Camiré, O., et al. Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices. J. Vis. Exp. (2018). This video demonstrates a protocol for whole-cell patch-clamp recording and two-photon calcium imaging in brain hippocampal slices. This method allows to study the dynamics of local Ca2+ transients (CaTs) in dendrites of different neuronal types in acute brain slices.

Publication Details

Journal: Journal of Visualized Experiments
Published: 2025-07-24
DOI: https://doi.org/10.3791/31175
Primary Topic: Biotin and Related Studies
Type: article
Field-Weighted Citation Impact: 0.00

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article

Establishing a Whole-Cell Configuration for Two-Photon Calcium Imaging of Brain Slices

Jul 24, 2025

Journal of Visualized Experiments

Biotin and Related Studies

article

Establishing a Whole-Cell Configuration for Two-Photon Calcium Imaging of Brain Slices

July 24, 2025article en

Abstract

Journal of Visualized Experiments

Zero hunger

Openalex Percentile: Top 80%

Biotin and Related Studies

DOI Source OpenAlex

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Establishing a Whole-Cell Configuration for Two-Photon Calcium Imaging of Brain Slices · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS