A Technique to Isolate Satellite Cells From Rat Head Muscle Tissue

Source: Carvajal Monroy, P. L., et al. Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles. J. Vis. Exp. (2015). This video demonstrates the isolation of satellite cells from rat head branchiomeric muscle tissues through tissue dissection, enzymatic digestion, and subsequent mechanical and centrifugal purification. The cells are then seeded on extracellular matrix-coated wells for differentiation into myoblasts, and eventually into myotubes and mature muscle fibers.