Obtaining a Mixed Glial Cell Culture from a Neonatal Mouse Brain

Source: Sarkar, S. et al., Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility. J. Vis. Exp. (2017) The video demonstrated the method of isolating and culturing cells from a neonatal mouse brain. It involved enzymatic and mechanical dissociation of the brain, followed by culturing in growth media lacking neuronal growth factor to obtain a mixed glial population.