siRNA-Mediated Gene Silencing in Neural Stem Cells

Source: Nguyen, B. H., et al. Culturing and Manipulation of O9-1 Neural Crest Cells. J. Vis. Exp (2018) This video demonstrates the use of small interfering RNA (siRNA) to silence specific genes of interest in neural stem cells and differentiate them into desired cell types using appropriate differentiation medium. This procedure enables the study of the critical role of specific genes in the differentiation process of neural stem cells.

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31049
Primary Topic
RNA Interference and Gene Delivery
Type
article
Field-Weighted Citation Impact
0.00
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article

siRNA-Mediated Gene Silencing in Neural Stem Cells

Journal of Visualized Experiments
RNA Interference and Gene Delivery
article

siRNA-Mediated Gene Silencing in Neural Stem Cells

article en

Abstract

Source: Nguyen, B. H., et al. Culturing and Manipulation of O9-1 Neural Crest Cells. J. Vis. Exp (2018) This video demonstrates the use of small interfering RNA (siRNA) to silence specific genes of interest in neural stem cells and differentiate them into desired cell types using appropriate differentiation medium. This procedure enables the study of the critical role of specific genes in the differentiation process of neural stem cells.

Journal of Visualized Experiments
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RNA Interference and Gene Delivery
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siRNA-Mediated Gene Silencing in Neural Stem Cells · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS