Generation of Medial Thalamus-Anterior Cingulate Cortex Slices from Mouse Brains

Source: Lu, H., et al. Direct-current Stimulation and Multi-electrode Array Recording of Seizure-like Activity in Mice Brain Slice Preparation. J. Vis. Exp. (2016) The video demonstrates the preparation of medial thalamus-anterior cingulate cortex (MT-ACC) slices from mouse brains. A mouse's brain is dissected, cuts are made to expose the MT-ACC pathway, and slices are generated using a vibratome. The MT-ACC slices are then maintained in oxygenated artificial cerebrospinal fluid.