Generating an Ultra-Low-Density Neuronal Culture Using a High-Density Neuronal Feeder Layer

Source: Lu, Z. et al., A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture. J. Vis. Exp. (2016). This video demonstrates the method for culturing ultra-low-density neurons in the presence of a high-density neuronal feeder layer. It establishes a co-culture of varying-density neurons, ensuring close physical proximity. The growth factors secreted by high-density neurons help neuronal survival and growth, maintaining ultra-low-density neurons for a longer time period.