A Technique for Harvesting and Dissociating Dorsal Root Ganglia for Neuronal Cultures

Source: de Luca, A. C., et al., Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration. J. Vis. Exp. (2015). This video demonstrates a method for harvesting neurons from the dorsal root ganglia (DRG). DRG from a rat's spinal column is isolated, treated with enzymes, and then mechanically dissociated to obtain a single-cell suspension. The neurons are separated from glial cells using density gradient centrifugation and cultured in a growth medium.