Growth and Differentiation of Magnetic Nano Particle-Loaded Neurons on Magnetic Platforms

Source: Indech, G., et al. Fabrication of Magnetic Platforms for Micron-Scale Organization of Interconnected Neurons. J. Vis. Exp. (2021). This video demonstrates the uptake of fluorescent magnetic nanoparticles (MNPs) by the neuronal cells via endocytosis. Growing these MNP-loaded neurons on the magnetically patterned substrate enables the attachment of the cells. The addition of a growth factor containing medium aids in the growth of neuron extensions, while the magnetic field of the substrate directs the orientation of the extension.