Generating Neurons and Glial Cells from Human Induced Pluripotent Stem Cell-Derived Embryoid Bodies

Source: Pistollato, F., et al. Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing. J. Vis. Exp.(2017) This video demonstrates the process of differentiating embryoid bodies (EBs) into neuroepithelial aggregates and their subsequent division into neuronal and glial cells. This method allows the efficient generation of neuronal and glial cells in vitro, which serve as test systems for toxicity testing as well as assessment of different pathways involved in neurotoxicity.