Preparation of Brain Sections Using a NMDG-Based Protective Recovery Method

Source: Ting, J. T. et al. Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method. J. Vis. Exp. (2018). This video describes a protocol to obtain brain slices using an N-Methyl-D-glucamine (NMDG)-based protective recovery method. A dissected brain is incubated in an aCSF with NMDG and HEPES to minimize neuronal cell death and damage during the sectioning. The section is allowed to recover in the aCSF to restore the cellular activities

Publication Details

Journal
Journal of Visualized Experiments
Published
2025-07-24
DOI
https://doi.org/10.3791/31023
Primary Topic
Anesthesia and Neurotoxicity Research
Type
article
Field-Weighted Citation Impact
0.00
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article

Preparation of Brain Sections Using a NMDG-Based Protective Recovery Method

Journal of Visualized Experiments
Anesthesia and Neurotoxicity Research
article

Preparation of Brain Sections Using a NMDG-Based Protective Recovery Method

article en

Abstract

Source: Ting, J. T. et al. Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method. J. Vis. Exp. (2018). This video describes a protocol to obtain brain slices using an N-Methyl-D-glucamine (NMDG)-based protective recovery method. A dissected brain is incubated in an aCSF with NMDG and HEPES to minimize neuronal cell death and damage during the sectioning. The section is allowed to recover in the aCSF to restore the cellular activities

Journal of Visualized Experiments
Openalex Percentile: Top 79%
Anesthesia and Neurotoxicity Research
DOISourceOpenAlex
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Preparation of Brain Sections Using a NMDG-Based Protective Recovery Method · Journal of Visualized Experiments (2025) | TGRS Research Map | TGRS