Generating Neural Progenitors from Mouse Embryonic Stem Cells by the Hanging Drop Method

Source: Hanafiah, A., et al. Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells. J. Vis. Exp. (2020). The video demonstrates the differentiation of mouse embryonic stem cells (mESCs) to embryoid bodies (EBs) by the hanging drop method. Upon suspending the mESCs in hanging drops and incubating, the cells aggregate to form EBs, which are further differentiated into neuronal progenitor cells (NPCs) using a differentiation medium supplemented with retinoic acid (RA).